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Glyko® Endoglycosidase H

Product Code:
PZGKE-5002
Our Price:
£216.00 GBP
Quantity:


Glyko® sequencing-grade Endo H

[endo-beta-Nacetylglucosaminidase H, EC 3.2.1.96] is isolated from a strain of E. coli expressing a cloned gene from Streptomyces plicatus.

Ships w/ Incubation Buffer & Denaturation Solution.


Applications:

  • Release of asparagine-linked oligomannose and hybrid, but not complex, oligosaccharides from glycopeptides and glycoproteins
  • Cell biology/protein intracellular trafficking studies
  • Therapeutic recombinant protein analysis
  • Glycan studies of therapeutic antibodies and process development changes
  • AIDS/HIV research
  • Functional role of glycans on proteins

 

Specificity:

Asparagine-linked hybrid or high-mannose oligosaccharides, either as free glycopeptides or as protein-linked glycans, are substrates for the enzyme. However, the enzyme does not cleave complex type glycans. Thus, Endo H is an extremely useful tool for delineating between these two types of N-linked structures on proteins.

In many cases, glycan removal with Endo H results in a significant reduction in apparent molecular weight of the protein substrate, leading to a detectable change in its electrophoretic migration when evalluated with SDS-PAGE.

The enzyme is also active against dolichollinked glycans containing these structures.
The presence of sulfate on oligomannose glycans may impair or reduce the rate of
enzyme cleavage.

 

Further information:

GKE-5002 Endo H Tech Sheet 031606AA.pdf

 

 

Tech Info:  [Endo-ß-N-acetylglucosaminidase H] cleaves between the N-acetylglucosamine residues of the chitobiose core of N-linked glycans. Will cleave oligomannose and most hybrid-type glycans, including those which are core fucosylated, whereas complex-type glycans are not hydrolyzed. insert specs Ships w/ Incubation Buffer & Denaturation Solution